Preparation of Low Serum for C2C12 cells

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Materials:

  • 390 mls DMEM-1 tube peni./ strepto.
  • tube glutamine (no insulin)
  • 8 mls Horse serum-1 sterile bottle
  • 500 ml sterile cylinder sterile filter 150 ml

    Procedure:

    1. Put 390 mls DMEM into the cylinder
    2. Than add Peni./Strepto. ,Glutamine (no insulin) Add 8 mls horse serum and than mix
    3. filtrate the media through a sterile filter into the sterile bottle
    4. Label the bottle with “C2C12, differentiation media” , name and date