Difference between revisions of "Preparation of Low Serum for C2C12 cells"
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| Line 1: | Line 1: | ||
| − | == Materials | + | == Materials == |
| − | + | * 390 mls DMEM-1 tube peni./ strepto. | |
| − | + | * tube glutamine | |
(no insulin) | (no insulin) | ||
| − | + | * 8 mls Horse serum-1 sterile bottle | |
| − | + | * 500 ml sterile cylinder | |
sterile filter 150 ml | sterile filter 150 ml | ||
| − | == Procedure | + | == Procedure == |
# Put 390 mls DMEM into the cylinder | # Put 390 mls DMEM into the cylinder | ||
# Than add Peni./Strepto. ,Glutamine (no insulin) Add 8 mls horse serum and than mix | # Than add Peni./Strepto. ,Glutamine (no insulin) Add 8 mls horse serum and than mix | ||
# filtrate the media through a sterile filter into the sterile bottle | # filtrate the media through a sterile filter into the sterile bottle | ||
# Label the bottle with “C2C12, differentiation media” , name and date | # Label the bottle with “C2C12, differentiation media” , name and date | ||
Revision as of 09:54, 27 March 2015
Materials
- 390 mls DMEM-1 tube peni./ strepto.
- tube glutamine
(no insulin)
- 8 mls Horse serum-1 sterile bottle
- 500 ml sterile cylinder
sterile filter 150 ml
Procedure
- Put 390 mls DMEM into the cylinder
- Than add Peni./Strepto. ,Glutamine (no insulin) Add 8 mls horse serum and than mix
- filtrate the media through a sterile filter into the sterile bottle
- Label the bottle with “C2C12, differentiation media” , name and date