Preparation of Low Serum for A1 cells

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  • 280 ml MEM
  • 100 ml sterile water
  • 1 tube Pen/ Strep (4ml)
  • 1 tube Glutamine (4ml)
  • 1 tube Insulin (4 ml)
  • 2 ml FCS
  • 500 ml filter with bottle


  1. put all together and filtrate all
  2. label all with Low Serum, name and date