Preparation of Low Serum for A1 cells

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Materials

- 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle

Procedure

- put all together and filtrate all - label all with Low Serum, name and date