Difference between revisions of "Preparation of Low Serum for A1 cells"
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(Created page with "== Materials == - 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle == Proce...") |
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| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | == | + | == Material == |
| − | + | * 280 ml MEM | |
| − | + | * 100 ml sterile water | |
| − | + | * 1 tube Pen/ Strep (4ml) | |
| − | + | * 1 tube Glutamine (4ml) | |
| − | + | * 1 tube Insulin (4 ml) | |
| − | + | * 2 ml FCS | |
| − | + | * 500 ml filter with bottle | |
== Procedure == | == Procedure == | ||
| − | + | # put all together and filtrate all | |
| − | + | # label all with Low Serum, name and date | |
Latest revision as of 14:26, 8 June 2015
Material
- 280 ml MEM
- 100 ml sterile water
- 1 tube Pen/ Strep (4ml)
- 1 tube Glutamine (4ml)
- 1 tube Insulin (4 ml)
- 2 ml FCS
- 500 ml filter with bottle
Procedure
- put all together and filtrate all
- label all with Low Serum, name and date