Difference between revisions of "Preparation of Low Serum for A1 cells"
Jump to navigation
Jump to search
| Line 9: | Line 9: | ||
== Procedure == | == Procedure == | ||
| − | + | # put all together and filtrate all | |
| − | + | # label all with Low Serum, name and date | |
Revision as of 12:45, 17 April 2015
Materials
- 280 ml MEM
- 100 ml sterile water
- 1 tube Pen/ Strep (4ml)
- 1 tube Glutamine (4ml)
- 1 tube Insulin (4 ml)
- 2 ml FCS
- 500 ml filter with bottle
Procedure
- put all together and filtrate all
- label all with Low Serum, name and date