Difference between revisions of "Preparation of Low Serum for A1 cells"

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(Created page with "== Materials == - 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle == Proce...")
 
(Materials)
Line 1: Line 1:
 
== Materials ==
 
== Materials ==
- 280 ml MEM
+
* 280 ml MEM
- 100 ml sterile water
+
* 100 ml sterile water
- 1 tube Pen/ Strep (4ml)
+
* 1 tube Pen/ Strep (4ml)
- 1 tube Glutamine (4ml)
+
* 1 tube Glutamine (4ml)
- 1 tube Insulin (4 ml)
+
* 1 tube Insulin (4 ml)
- 2 ml FCS
+
* 2 ml FCS
- 500 ml filter with bottle
+
* 500 ml filter with bottle
  
 
== Procedure ==
 
== Procedure ==
 
- put all together and filtrate all  
 
- put all together and filtrate all  
 
- label all with Low Serum, name and date
 
- label all with Low Serum, name and date

Revision as of 12:44, 17 April 2015

Materials

  • 280 ml MEM
  • 100 ml sterile water
  • 1 tube Pen/ Strep (4ml)
  • 1 tube Glutamine (4ml)
  • 1 tube Insulin (4 ml)
  • 2 ml FCS
  • 500 ml filter with bottle

Procedure

- put all together and filtrate all - label all with Low Serum, name and date