Preparation of Freezing media for C2C12 cells

From Tanaka Wiki
Revision as of 08:50, 25 March 2015 by Stephanh (talk | contribs) (Created page with "Materials: 25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro Procedure: - put all stuffs together - filtate the mediu...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Materials:

25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro


Procedure:

- put all stuffs together - filtate the medium and aliquote the freezing media into 4 ml per tube - store at –20ºC