Difference between revisions of "Preparation of Freezing media for C2C12 cells"
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(Created page with "Materials: 25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro Procedure: - put all stuffs together - filtate the mediu...") |
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| − | + | == Material == | |
| − | 25 ml FCS (50%) | + | * 25 ml FCS (50%) |
| − | 20 ml DMEM + Pen/ Strep + Glut (40%) | + | * 20 ml DMEM + Pen/ Strep + Glut (40%) |
| − | 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro | + | * 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro |
| − | Procedure | + | == Procedure == |
| − | + | # put all stuffs together | |
| − | + | # filtate the medium and aliquote the freezing media into 4 ml per tube | |
| − | + | # store at –20ºC | |
Latest revision as of 14:34, 8 June 2015
Material
- 25 ml FCS (50%)
- 20 ml DMEM + Pen/ Strep + Glut (40%)
- 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro
Procedure
- put all stuffs together
- filtate the medium and aliquote the freezing media into 4 ml per tube
- store at –20ºC