Difference between revisions of "Preparation of Freezing media for A1 cells"
Jump to navigation
Jump to search
(Created page with "Materials * DMSO (one ampules from Sigma) * High serum for A1 cells * 2x 50 ml FALCON tubes * 1x 15 ml FALCON tube * Pipettes ** 10x 5 ml FALCON tubes Procedure - clean the a...") |
|||
| Line 1: | Line 1: | ||
| − | Materials | + | == Materials == |
* DMSO (one ampules from Sigma) | * DMSO (one ampules from Sigma) | ||
* High serum for A1 cells | * High serum for A1 cells | ||
| Line 5: | Line 5: | ||
* 1x 15 ml FALCON tube | * 1x 15 ml FALCON tube | ||
* Pipettes | * Pipettes | ||
| − | + | * 10x 5 ml FALCON tubes | |
| − | Procedure | + | == Procedure == |
- clean the ampuls with ethanol | - clean the ampuls with ethanol | ||
- transfer the DMSO into the 15 ml FALCON tube | - transfer the DMSO into the 15 ml FALCON tube | ||
Revision as of 12:54, 17 April 2015
Materials
- DMSO (one ampules from Sigma)
- High serum for A1 cells
- 2x 50 ml FALCON tubes
- 1x 15 ml FALCON tube
- Pipettes
- 10x 5 ml FALCON tubes
Procedure
- clean the ampuls with ethanol - transfer the DMSO into the 15 ml FALCON tube - pipette in each 50 ml FALCON tube 18 ml High serum - put into each 2 ml DMSO and mix - aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube) - store the tubes into –20ºC