Difference between revisions of "Preparation of Freezing media for A1 cells"
Jump to navigation
Jump to search
| Line 1: | Line 1: | ||
| − | == | + | == Material == |
* DMSO (one ampules from Sigma) | * DMSO (one ampules from Sigma) | ||
* High serum for A1 cells | * High serum for A1 cells | ||
Latest revision as of 14:28, 8 June 2015
Material
- DMSO (one ampules from Sigma)
- High serum for A1 cells
- 2x 50 ml FALCON tubes
- 1x 15 ml FALCON tube
- Pipettes
- 10x 5 ml FALCON tubes
Procedure
- clean the ampuls with ethanol
- transfer the DMSO into the 15 ml FALCON tube
- pipette in each 50 ml FALCON tube 18 ml High serum
- put into each 2 ml DMSO and mix
- aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube)
- store the tubes into –20ºC