Difference between revisions of "Preparation of Freezing media for A1 cells"
Jump to navigation
Jump to search
(Created page with "Materials * DMSO (one ampules from Sigma) * High serum for A1 cells * 2x 50 ml FALCON tubes * 1x 15 ml FALCON tube * Pipettes ** 10x 5 ml FALCON tubes Procedure - clean the a...") |
|||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | + | == Material == | |
* DMSO (one ampules from Sigma) | * DMSO (one ampules from Sigma) | ||
* High serum for A1 cells | * High serum for A1 cells | ||
| Line 5: | Line 5: | ||
* 1x 15 ml FALCON tube | * 1x 15 ml FALCON tube | ||
* Pipettes | * Pipettes | ||
| − | + | * 10x 5 ml FALCON tubes | |
| − | Procedure | + | == Procedure == |
| − | + | # clean the ampuls with ethanol | |
| − | + | # transfer the DMSO into the 15 ml FALCON tube | |
| − | + | # pipette in each 50 ml FALCON tube 18 ml High serum | |
| − | + | # put into each 2 ml DMSO and mix | |
| − | + | # aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube) | |
| − | + | # store the tubes into –20ºC | |
Latest revision as of 14:28, 8 June 2015
Material
- DMSO (one ampules from Sigma)
- High serum for A1 cells
- 2x 50 ml FALCON tubes
- 1x 15 ml FALCON tube
- Pipettes
- 10x 5 ml FALCON tubes
Procedure
- clean the ampuls with ethanol
- transfer the DMSO into the 15 ml FALCON tube
- pipette in each 50 ml FALCON tube 18 ml High serum
- put into each 2 ml DMSO and mix
- aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube)
- store the tubes into –20ºC