Difference between revisions of "Preparation of Freezing media for A1 cells"
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| Line 8: | Line 8: | ||
== Procedure == | == Procedure == | ||
| − | + | # clean the ampuls with ethanol | |
| − | + | # transfer the DMSO into the 15 ml FALCON tube | |
| − | + | # pipette in each 50 ml FALCON tube 18 ml High serum | |
| − | + | # put into each 2 ml DMSO and mix | |
| − | + | # aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube) | |
| − | + | # store the tubes into –20ºC | |
Revision as of 12:58, 17 April 2015
Materials
- DMSO (one ampules from Sigma)
- High serum for A1 cells
- 2x 50 ml FALCON tubes
- 1x 15 ml FALCON tube
- Pipettes
- 10x 5 ml FALCON tubes
Procedure
- clean the ampuls with ethanol
- transfer the DMSO into the 15 ml FALCON tube
- pipette in each 50 ml FALCON tube 18 ml High serum
- put into each 2 ml DMSO and mix
- aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube)
- store the tubes into –20ºC