Difference between revisions of "LiberaseTM dissociation"
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| Line 6: | Line 6: | ||
*dH2O 15 mL | *dH2O 15 mL | ||
*Total 50 mL | *Total 50 mL | ||
| − | + | Store at room temperature. Chill on ice before use. | |
Revision as of 20:20, 18 July 2022
Preparing reagents
0.7x Phosphate Buffer Saline (PBS)
- 1x PBS 35 mL
- dH2O 15 mL
- Total 50 mL
Store at room temperature. Chill on ice before use.
Stock Liberase TM (26 Wünsch Unit (WU)/mL)
- Liberase TM 50mg
- dH2O 10mL
- Store in 50uL aliquots at -80 degree