Difference between revisions of "LiberaseTM dissociation"
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| Line 2: | Line 2: | ||
---- | ---- | ||
| − | + | '''0.7x Phosphate Buffer Saline (PBS)''' | |
| − | 1x PBS 35 mL | + | *1x PBS 35 mL |
| − | dH2O 15 mL | + | *dH2O 15 mL |
| − | Total 50 mL | + | *Total 50 mL |
| − | Store at room temperature. Chill on ice before use. | + | *Store at room temperature. Chill on ice before use. |
'''Stock Liberase TM (26 Wünsch Unit (WU)/mL)''' | '''Stock Liberase TM (26 Wünsch Unit (WU)/mL)''' | ||
| − | Liberase TM 50mg | + | *Liberase TM 50mg |
| − | dH2O 10mL | + | *dH2O 10mL |
| − | Store in 50uL aliquots at -80 degree | + | *Store in 50uL aliquots at -80 degree |
Revision as of 20:19, 18 July 2022
Preparing reagents
0.7x Phosphate Buffer Saline (PBS)
- 1x PBS 35 mL
- dH2O 15 mL
- Total 50 mL
- Store at room temperature. Chill on ice before use.
Stock Liberase TM (26 Wünsch Unit (WU)/mL)
- Liberase TM 50mg
- dH2O 10mL
- Store in 50uL aliquots at -80 degree