Difference between revisions of "LiberaseTM dissociation"
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= Preparing reagents = | = Preparing reagents = | ||
---- | ---- | ||
| + | |||
| + | '''0.7x Phosphate Buffer Saline (PBS)''' | ||
| + | 1x PBS 35 mL | ||
| + | dH2O 15 mL | ||
| + | Total 50 mL | ||
| + | Store at room temperature. Chill on ice before use. | ||
| + | |||
| + | '''Stock Liberase TM (26 Wünsch Unit (WU)/mL)''' | ||
| + | Liberase TM 50mg | ||
| + | dH2O 10mL | ||
| + | Store in 50uL aliquots at -80 degree | ||
Revision as of 20:17, 18 July 2022
Preparing reagents
0.7x Phosphate Buffer Saline (PBS) 1x PBS 35 mL dH2O 15 mL Total 50 mL Store at room temperature. Chill on ice before use.
Stock Liberase TM (26 Wünsch Unit (WU)/mL) Liberase TM 50mg dH2O 10mL Store in 50uL aliquots at -80 degree