Difference between revisions of "Labeled AB staining"
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10 µl NeuN (0.5 mg/ml)
11,8 µl cy5 (0.85 mg/ml)
80 µl PBS/ BSA (1mg/ml BSA in PBS → sterile filter)
30 min @ RT in alu foil
12 µl α - BrdU → 30 min @ RT in alu foil
400 µl TBS – Tween + 10% goat serum
(Created page with "<h4>Pax7 + cy3 + NeuN – cy5Fab</h4>") |
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<h4>Pax7 + cy3 + NeuN – cy5Fab</h4> | <h4>Pax7 + cy3 + NeuN – cy5Fab</h4> | ||
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| + | <h3>NeuN – cy5Fab cocktail for 2 slides</h3> | ||
| + | <li>10 µl NeuN (0.5 mg/ml)</li> | ||
| + | <li>11,8 µl cy5 (0.85 mg/ml)</li> | ||
| + | <li>80 µl PBS/ BSA (1mg/ml BSA in PBS → sterile filter)</li> | ||
| + | <li>30 min @ RT in alu foil</li> | ||
| + | <li>12 µl α - BrdU → 30 min @ RT in alu foil</li> | ||
| + | <li>400 µl TBS – Tween + 10% goat serum</li> | ||