G418 medium preparation
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Material
- 2x 15 ml FALCON tube
- filter 0.22 µl
- 5 ml syringe
- DMEM
- G418
Procedure
- 1 g of weight has a total concentration from 0,751 g
- How much of DMEM we need for 1,5 g G418 if we prepare a stock solution with a concentration of 200 mg/ ml?
- 1.5g powder has a total concentration from 1,1256 g G418
1125,6 mg/ 200 mg = 5,6
it means, that you need 5,6 ml DMEM for 1,5 g G418 , if you wanted a concentration of 200 mg/ ml
- dissolve the powder in DMEM
- if it is dissolve than filter the solution:
put the pump from the syringe out, fill the antibiotic in the syringe, take the pump back and press all across the filter
- label the vessel with the name of the antibiotics and the stock solution
f.e. G418 200 mg/ ml in DMEM
for using:
HS with antibiotics: 400 µg/ ml
- 0,2/ 0,0004 = 500 ⇒ 100 ml media/ 500 ⇒ 0,2 ml antibiotics in 1200 ml media