Difference between revisions of "G418 medium preparation"
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2x 15 ml FALCON tube
filter 0.22 µl
5 ml syringe
DMEM
G418
dissolve the powder in DMEM
if it is dissolve than filter the solution:
put the pump from the syringe out, fill the antibiotic in the syringe, take the pump back and press all across the filter
label the vessel with the name of the antibiotics and the stock solution
f.e. G418 200 mg/ ml in DMEM
for using:
HS with antibiotics: 400 µg/ ml
0,2/ 0,0004 = 500 ⇒ 100 ml media/ 500 ⇒ 0,2 ml antibiotics in 1200 ml media
| Line 15: | Line 15: | ||
it means, that you need 5,6 ml DMEM for 1,5 g G418 , if you wanted a concentration of 200 mg/ ml | it means, that you need 5,6 ml DMEM for 1,5 g G418 , if you wanted a concentration of 200 mg/ ml | ||
| − | + | <li>dissolve the powder in DMEM | |
| − | + | <li>if it is dissolve than filter the solution: | |
put the pump from the syringe out, fill the antibiotic in the syringe, take the pump back and press all across the filter | put the pump from the syringe out, fill the antibiotic in the syringe, take the pump back and press all across the filter | ||
| − | + | <li>label the vessel with the name of the antibiotics and the stock solution | |
f.e. G418 200 mg/ ml in DMEM | f.e. G418 200 mg/ ml in DMEM | ||
Revision as of 09:37, 15 August 2014
Materials:
Execution:
1 g of weight has a total concentration from 0,751 g How much of DMEM we need for 1,5 g G418 if we prepare a stock solution with a concentration of 200 mg/ ml? 1.5g powder has a total concentration from 1,1256 g G418
1125,6 mg/ 200 mg = 5,6
it means, that you need 5,6 ml DMEM for 1,5 g G418 , if you wanted a concentration of 200 mg/ ml