Difference between revisions of "G418 medium preparation"
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- 2x 15 ml FALCON tube | - 2x 15 ml FALCON tube | ||
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- G418 | - G418 | ||
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1 g of weight has a total concentration from 0,751 g | 1 g of weight has a total concentration from 0,751 g | ||
How much of DMEM we need for 1,5 g G418 if we prepare a stock solution with a concentration of 200 mg/ ml? | How much of DMEM we need for 1,5 g G418 if we prepare a stock solution with a concentration of 200 mg/ ml? | ||
Revision as of 09:33, 15 August 2014
Materials:
- 2x 15 ml FALCON tube - filter 0.22 µl - 5 ml syringe - DMEM - G418
Execution:
1 g of weight has a total concentration from 0,751 g How much of DMEM we need for 1,5 g G418 if we prepare a stock solution with a concentration of 200 mg/ ml? 1.5g powder has a total concentration from 1,1256 g G418
1125,6 mg/ 200 mg = 5,6
it means, that you need 5,6 ml DMEM for 1,5 g G418 , if you wanted a concentration of 200 mg/ ml
- dissolve the powder in DMEM - if it is dissolve than filter the solution: put the pump from the syringe out, fill the antibiotic in the syringe, take the pump back and press all across the filter - label the vessel with the name of the antibiotics and the stock solution f.e. G418 200 mg/ ml in DMEM
for using: HS with antibiotics: 400 µg/ ml 0,2/ 0,0004 = 500 ⇒ 100 ml media/ 500 ⇒ 0,2 ml antibiotics in 1200 ml media