Difference between revisions of "BrdU labelling of A1 cells in 96-well plate"
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| Line 1: | Line 1: | ||
| − | == Materials | + | == Materials == |
* 5ml tube | * 5ml tube | ||
* SF MEM | * SF MEM | ||
| Line 7: | Line 7: | ||
* Pipette 5ml | * Pipette 5ml | ||
| − | == | + | == Procedure == |
# put 2 mls SF MEM into the 5 ml tube | # put 2 mls SF MEM into the 5 ml tube | ||
# add 40ul BrDU | # add 40ul BrDU | ||
Revision as of 09:39, 25 March 2015
Materials
- 5ml tube
- SF MEM
- BrDU drawer no. 3
- Multipipette
- Pipette 100ul
- Pipette 5ml
Procedure
- put 2 mls SF MEM into the 5 ml tube
- add 40ul BrDU
- mix the liquid
- put 10 ul into each well , use the multipipette
- incubate for 6-8 h or overnight(then you can fix cells for stain)
- label the 96 well plate with BrDU and date