Difference between revisions of "BrdU labelling of A1 cells in 96-well plate"
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(Created page with "== Materials: == * 5ml tube * SF MEM * BrDU drawer no. 3 * Multipipette * Pipette 100ul * Pipette 5ml") |
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| Line 1: | Line 1: | ||
| − | == | + | == Material == |
* 5ml tube | * 5ml tube | ||
* SF MEM | * SF MEM | ||
| Line 6: | Line 6: | ||
* Pipette 100ul | * Pipette 100ul | ||
* Pipette 5ml | * Pipette 5ml | ||
| + | |||
| + | == Procedure == | ||
| + | # put 2 mls SF MEM into the 5 ml tube | ||
| + | # add 40ul BrDU | ||
| + | # mix the liquid | ||
| + | # put 10 ul into each well , use the multipipette | ||
| + | # incubate for 6-8 h or overnight(then you can fix cells for stain) | ||
| + | # label the 96 well plate with BrDU and date | ||
Latest revision as of 14:25, 8 June 2015
Material
- 5ml tube
- SF MEM
- BrDU drawer no. 3
- Multipipette
- Pipette 100ul
- Pipette 5ml
Procedure
- put 2 mls SF MEM into the 5 ml tube
- add 40ul BrDU
- mix the liquid
- put 10 ul into each well , use the multipipette
- incubate for 6-8 h or overnight(then you can fix cells for stain)
- label the 96 well plate with BrDU and date