Difference between revisions of "Benzocaine"
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<li>Fill up with destilled water</li> | <li>Fill up with destilled water</li> | ||
</ul> | </ul> | ||
| + | |||
| + | === Stock solutions === | ||
Usually you can find all stock solutions are at Maritta's bench. | Usually you can find all stock solutions are at Maritta's bench. | ||
However, if for some reasons they are not there here are the recipes. | However, if for some reasons they are not there here are the recipes. | ||
| − | === 10x TBS === | + | ==== 10x TBS ==== |
Mix the following components with stir bar: | Mix the following components with stir bar: | ||
<ul> | <ul> | ||
| Line 24: | Line 26: | ||
Afterwards add 10 ml of concentrated HCL (12M) and check pH for pH (it should be 8). | Afterwards add 10 ml of concentrated HCL (12M) and check pH for pH (it should be 8). | ||
| − | === 10% Benzocaine Stock === | + | ==== 10% Benzocaine Stock ==== |
10% w/v benzocaine (chemical powder above Maritta's bench) | 10% w/v benzocaine (chemical powder above Maritta's bench) | ||
Fill up with 100% EtOH. | Fill up with 100% EtOH. | ||
| − | === 4000% Holtfreter === | + | ==== 4000% Holtfreter ==== |
This is provided by the axolotl colony. | This is provided by the axolotl colony. | ||
Latest revision as of 15:10, 7 November 2012
Responsible persons
If you have any troubles please contact Maritta or Eugen.
Recipe
For 0.03% benzocaine you need the following:
- 500ml 10xTBS
- 50ml 4000% Holtfreter
- 30ml 10% Benzocaine stock
- Fill up with destilled water
Stock solutions
Usually you can find all stock solutions are at Maritta's bench. However, if for some reasons they are not there here are the recipes.
10x TBS
Mix the following components with stir bar:
- 24.2g Tris base
- 90g NaCl
- 990 ml distilled water
Afterwards add 10 ml of concentrated HCL (12M) and check pH for pH (it should be 8).
10% Benzocaine Stock
10% w/v benzocaine (chemical powder above Maritta's bench) Fill up with 100% EtOH.
4000% Holtfreter
This is provided by the axolotl colony.