For scRNA-seq

scRNA-seq

---

(Continue from the dissociation protocol)

• After 30um filter cup, immediately filter through 10um filter cup

Resuspend the cell pellet with 150-300uL AMEM(0) depending on the size of the pellet.

• Immediately sort the cells into 1.5mL tube with 700uL AMEM(0).
  • Normally we use F02 sorter because it has lasers for both GFP and mCherry.
• Note the FACS count.
• Centrifuge 300xrcf, 20 degree, 5 minutes. Remove supernatant.

• The sample is ready for downstream applications:

-> scRNA-seq

-> cell transplantation