Fibronectin preparation

Materials;

• Fibronectin from bovine plasma (SIGMA, Cat#: F4759-5MG); 5mg

ddH2O; 5ml

• Filter for syringe (0.22 µm); 1
• Syringe (10ml or bigger)
• 15ml Conical tube (Corning, Cat#: 430791); 1
• Eppendorf tube (1.5ml); 25
• Water bath at 37ºC

Method:

1. Pre-warm the water in the water bath for 10 min
2. Add the water directly to the fibronectin vial under the tissue culture hood
3. Incubate the vial in the water bath for 30 min and mix it time to time

• The procedure has to be done under the tissue culture hood from the step 4 and beyond

1. Set the filter to the syringe and put the fibronectin into the syringe
2. Filter it in 15ml conical tube
3. Aliquot the fibronectin, 200 µl/tube
4. Store at -20ºC