G418 medium preparation

Materials

• 2x 15 ml FALCON tube
• filter 0.22 µl
• 5 ml syringe
• DMEM
• G418

Procedure

1. 1 g of weight has a total concentration from 0,751 g
2. How much of DMEM we need for 1,5 g G418 if we prepare a stock solution with a concentration of 200 mg/ ml?
3. 1.5g powder has a total concentration from 1,1256 g G418

1125,6 mg/ 200 mg = 5,6

it means, that you need 5,6 ml DMEM for 1,5 g G418 , if you wanted a concentration of 200 mg/ ml

• dissolve the powder in DMEM
• if it is dissolve than filter the solution:

put the pump from the syringe out, fill the antibiotic in the syringe, take the pump back and press all across the filter

• label the vessel with the name of the antibiotics and the stock solution

f.e. G418 200 mg/ ml in DMEM

for using:

HS with antibiotics: 400 µg/ ml

• 0,2/ 0,0004 = 500 ⇒ 100 ml media/ 500 ⇒ 0,2 ml antibiotics in 1200 ml media