G418 medium preparation

Materials: - 2x 15 ml FALCON tube - filter 0.22 µl - 5 ml syringe - DMEM - G418

Execution: 1 g of weight has a total concentration from 0,751 g How much of DMEM we need for 1,5 g G418 if we prepare a stock solution with a concentration of 200 mg/ ml? 1.5g powder has a total concentration from 1,1256 g G418

1125,6 mg/ 200 mg = 5,6

it means, that you need 5,6 ml DMEM for 1,5 g G418 , if you wanted a concentration of 200 mg/ ml

- dissolve the powder in DMEM - if it is dissolve than filter the solution: put the pump from the syringe out, fill the antibiotic in the syringe, take the pump back and press all across the filter - label the vessel with the name of the antibiotics and the stock solution f.e. G418 200 mg/ ml in DMEM

for using: HS with antibiotics: 400 µg/ ml 0,2/ 0,0004 = 500 ⇒ 100 ml media/ 500 ⇒ 0,2 ml antibiotics in 1200 ml media