Materials:
1 x 15 ml conical tube per vial of frozen cells
water bath (37 C for mammalian cells)
High serum media (RPMI media +10% FCS+glutamine+P/S) [****Use Defined Supplemented Calf Serum (DSCS) from Hyclone (Prebio) for making high serum media for hybridoma cells]
1 blue cap flask
1 x 10 ml pipettes
gestopfft pasteur pipettes
ungestopfft pasteur pipettes
frozen cells in liquid nitrogen or dry ice.