Purifying IgG1 from Hybridoma Supernatant

Materials:

Supernatant (100-200 ml)

Peristaltic pump

1 ml HiTrap ProteinG column (binding capacity = 25 ml)

PBS (100 ml)

10x PBS

.1 M glycine pH 2.7 (25 ml)

1 M Tris pH 9.0 (10 ml)

10 Eppendorf tubes

20% ETOH/H2O (25 ml)