Pax7 + cy3 + NeuN – cy5Fab
NeuN – cy5Fab cocktail for 2 slides
10 µl NeuN (0.5 mg/ml)
11,8 µl cy5 (0.85 mg/ml)
80 µl PBS/ BSA (1mg/ml BSA in PBS → sterile filter)
30 min @ RT in alu foil
12 µl α - BrdU → 30 min @ RT in alu foil
400 µl TBS – Tween + 10% goat serum