User contributions for Stephanh

26 September 2014

• 08:0708:07, 26 September 2014 diff hist +6‎ Passaging A1 cell ‎No edit summary
• 08:0608:06, 26 September 2014 diff hist +787‎ Passaging A1 cell ‎No edit summary

24 September 2014

• 07:4407:44, 24 September 2014 diff hist +4‎ Thawing frozen A1 cells ‎No edit summary
• 07:4307:43, 24 September 2014 diff hist 0‎ Thawing frozen A1 cells ‎No edit summary
• 07:3707:37, 24 September 2014 diff hist +828‎ N Thawing frozen A1 cells ‎ Created page with "== Materials: == - Gelatin (warmed to 37ºC) - HS for A1 cells - 1x 15 ml FALCON tubes - Pipettes - 1x 175 cm2 flasks - frozen cells into liquid nitrogen == Procedure: == - p..."

5 September 2014

• 08:4508:45, 5 September 2014 diff hist +2‎ Electroporation of A1 cells ‎No edit summary
• 08:4508:45, 5 September 2014 diff hist +15‎ Electroporation of A1 cells ‎No edit summary
• 08:4308:43, 5 September 2014 diff hist +11‎ Electroporation of A1 cells ‎No edit summary
• 08:4108:41, 5 September 2014 diff hist +14‎ Electroporation of A1 cells ‎No edit summary
• 08:3608:36, 5 September 2014 diff hist 0‎ Electroporation of A1 cells ‎No edit summary
• 08:3508:35, 5 September 2014 diff hist +7‎ Electroporation of A1 cells ‎No edit summary
• 08:1608:16, 5 September 2014 diff hist −3‎ Electroporation of A1 cells ‎No edit summary
• 08:1408:14, 5 September 2014 diff hist −4‎ Electroporation of A1 cells ‎No edit summary
• 08:1208:12, 5 September 2014 diff hist +100‎ Electroporation of A1 cells ‎No edit summary
• 07:2607:26, 5 September 2014 diff hist +3‎ Electroporation of A1 cells ‎No edit summary
• 07:2407:24, 5 September 2014 diff hist 0‎ Electroporation of A1 cells ‎No edit summary
• 07:2307:23, 5 September 2014 diff hist −5‎ Electroporation of A1 cells ‎No edit summary
• 07:2207:22, 5 September 2014 diff hist −13‎ Electroporation of A1 cells ‎No edit summary
• 07:2007:20, 5 September 2014 diff hist +12‎ Electroporation of A1 cells ‎No edit summary
• 07:1907:19, 5 September 2014 diff hist +4‎ Electroporation of A1 cells ‎No edit summary
• 07:1807:18, 5 September 2014 diff hist +899‎ N Electroporation of A1 cells ‎ Created page with "==Materials:== Ice 4 mM cuvettes, N+1 cuvettes Steinberg's, at 4 C. Square pulse electroporator (we use the BTX 830 Squarporator) 1) Put cuvettes on ice, and cool down..."

3 September 2014

• 09:3309:33, 3 September 2014 diff hist 0‎ Thawing frozen C2C12 cells ‎No edit summary
• 09:3109:31, 3 September 2014 diff hist +711‎ N Thawing frozen C2C12 cells ‎ Created page with "==Materials:== - frozen C2C12 cells - HS for C2C12 cells - pipettes and tips - FALCON tube 15 ml - Cell culture flask ==Procedure:== - heat the HS up to 37ºC - pipette 14 ml..."
• 09:0909:09, 3 September 2014 diff hist 0‎ Myotube preparation of C2C12 cells ‎No edit summary
• 09:0509:05, 3 September 2014 diff hist 0‎ Myotube preparation of C2C12 cells ‎No edit summary
• 09:0309:03, 3 September 2014 diff hist +8‎ Myotube preparation of C2C12 cells ‎No edit summary
• 09:0209:02, 3 September 2014 diff hist +2,773‎ N Myotube preparation of C2C12 cells ‎ Created page with "Materials for 1 x 96 well plate: - DMEM (3.5 ml) –purely - Fibronectin (final diluted concentration should be 20 ng/µl)) - 1x 96 well plate - 2% Horse Serum – Low Serum (..."

29 August 2014

• 07:1707:17, 29 August 2014 diff hist −6‎ Whole mount ISH on axolotl tissue ‎No edit summary
• 07:1507:15, 29 August 2014 diff hist −24‎ Whole mount ISH on axolotl tissue ‎No edit summary
• 07:1407:14, 29 August 2014 diff hist +1‎ Whole mount ISH on axolotl tissue ‎No edit summary
• 07:1407:14, 29 August 2014 diff hist +3‎ Whole mount ISH on axolotl tissue ‎No edit summary
• 07:1307:13, 29 August 2014 diff hist +1‎ Whole mount ISH on axolotl tissue ‎No edit summary
• 07:1207:12, 29 August 2014 diff hist +19‎ Whole mount ISH on axolotl tissue ‎No edit summary
• 07:0807:08, 29 August 2014 diff hist +3‎ Whole mount ISH on axolotl tissue ‎No edit summary
• 07:0607:06, 29 August 2014 diff hist +6‎ Whole mount ISH on axolotl tissue ‎No edit summary
• 07:0407:04, 29 August 2014 diff hist −40‎ Whole mount ISH on axolotl tissue ‎No edit summary
• 07:0007:00, 29 August 2014 diff hist +3,046‎ N Whole mount ISH on axolotl tissue ‎ Created page with "# fix embryo or tail blastema in MEMFA (0.1M MOPS pH7.4, 2mM EGTA, 1mM MgSO4, 3.7% formaldehyde) 2h to over night at room temperature # store at –20C in 100% MeOH or EtOH (p..."

22 August 2014

• 10:2810:28, 22 August 2014 diff hist +835‎ N Labelling Pax6 with Dig-NHS ‎ Created page with "2 June 2005 Pax6 AB is at 5 mg/ml, 1 ml 1) Dialyze 500 µl into PBS O/N then one change in morning 2 hours. 2) Spin antibody and check protein concentration by BioRad STD ..."
• 08:0208:02, 22 August 2014 diff hist +22‎ Thawing 293FT cells ‎No edit summary
• 07:5607:56, 22 August 2014 diff hist −9‎ Thawing 293FT cells ‎No edit summary
• 07:5207:52, 22 August 2014 diff hist +846‎ N Thawing 293FT cells ‎ Created page with "→ thaw the cells in prewarmed , complete medium without Geneticin materials: - a vial of frozen cells from the liquid nitrogen - 37ºC waterbath - 75 cm2 flask - HS for 293..."

15 August 2014

• 09:4309:43, 15 August 2014 diff hist +6‎ G418 medium preparation ‎No edit summary
• 09:4109:41, 15 August 2014 diff hist +4‎ G418 medium preparation ‎No edit summary
• 09:3909:39, 15 August 2014 diff hist +9‎ G418 medium preparation ‎No edit summary
• 09:3709:37, 15 August 2014 diff hist +6‎ G418 medium preparation ‎No edit summary
• 09:3509:35, 15 August 2014 diff hist +10‎ G418 medium preparation ‎No edit summary
• 09:3309:33, 15 August 2014 diff hist +6‎ G418 medium preparation ‎No edit summary
• 09:3009:30, 15 August 2014 diff hist +878‎ N G418 medium preparation ‎ Created page with "'''Materials:''' - 2x 15 ml FALCON tube - filter 0.22 µl - 5 ml syringe - DMEM - G418 '''Execution:''' 1 g of weight has a total concentration from 0,751 g How much of DMEM ..."

11 August 2014

• 08:0108:01, 11 August 2014 diff hist −11‎ Cardiomyocyte Preparation ‎No edit summary
• 08:0008:00, 11 August 2014 diff hist +15‎ Cardiomyocyte Preparation ‎No edit summary