User contributions for Stephanh
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11 June 2014
- 08:1708:17, 11 June 2014 diff hist +20 BrdU and myosin staining →For staining cells:
- 08:1508:15, 11 June 2014 diff hist +19 BrdU and myosin staining →Materials:
- 08:0908:09, 11 June 2014 diff hist −15 BrdU and myosin staining No edit summary
- 08:0108:01, 11 June 2014 diff hist −14 BrdU and myosin staining No edit summary
- 07:5907:59, 11 June 2014 diff hist −7 BrdU and myosin staining No edit summary
- 07:5607:56, 11 June 2014 diff hist −2 BrdU and myosin staining No edit summary
- 07:5507:55, 11 June 2014 diff hist +3,204 N BrdU and myosin staining Created page with "== Fixing Cells: == 1 thaw 6% PFA in 37 C waterbath , place on ice 2 put 5 ml PBS + 5 ml 6% PFA in tray “PFA” , mix well 3 put 0.01% BSA/PBS into tray “BSA” ..."
7 May 2014
- 08:2708:27, 7 May 2014 diff hist +943 N Antibody staining Created page with "* Cut the tail and fix it in fresh 4% PFA in phosphate buffer from few hours to o/n always at 4C * Wash in PBS 1 hour at room temperature, put tail in 30% sucrose Put in OCT s..."
2 May 2014
- 09:0009:00, 2 May 2014 diff hist +252 BrdU labelling of A1 cells in 96-well plate No edit summary
- 08:5608:56, 2 May 2014 diff hist +102 N BrdU labelling of A1 cells in 96-well plate Created page with "== Materials: == * 5ml tube * SF MEM * BrDU drawer no. 3 * Multipipette * Pipette 100ul * Pipette 5ml"
28 April 2014
- 08:4608:46, 28 April 2014 diff hist 0 Passaging C2C12 cells No edit summary
- 08:4508:45, 28 April 2014 diff hist 0 Passaging C2C12 cells No edit summary
- 08:3608:36, 28 April 2014 diff hist 0 Passaging C2C12 cells No edit summary
- 08:3408:34, 28 April 2014 diff hist 0 Passaging C2C12 cells No edit summary
- 08:3208:32, 28 April 2014 diff hist 0 Passaging C2C12 cells No edit summary
- 08:2908:29, 28 April 2014 diff hist +805 Passaging C2C12 cells No edit summary
- 08:2808:28, 28 April 2014 diff hist +17 Passaging C2C12 cells No edit summary
- 06:5806:58, 28 April 2014 diff hist +143 N Passaging C2C12 cells Created page with "== Materials: == * 1x 15ml FALCON tube * PBS w/o Ca2+ and Mg2+ * TE in PBS (4 ml TE + 36 ml PBS) * HS for C2C12 cells * pipettes * 75 cm2 flask"
24 March 2014
- 09:3209:32, 24 March 2014 diff hist +768 Purifying IgG1 from Hybridoma Supernatant No edit summary
- 09:2709:27, 24 March 2014 diff hist −45 Purifying IgG1 from Hybridoma Supernatant No edit summary
- 09:2509:25, 24 March 2014 diff hist +313 N Purifying IgG1 from Hybridoma Supernatant Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ..."
18 March 2014
- 12:4812:48, 18 March 2014 diff hist −6 Overview →Protocols
- 12:4512:45, 18 March 2014 diff hist +6 Overview →Antibody
- 12:4212:42, 18 March 2014 diff hist +133 Overview →Protocols
- 12:1912:19, 18 March 2014 diff hist +861 Labeled AB staining No edit summary
- 12:1212:12, 18 March 2014 diff hist +315 Labeled AB staining No edit summary
- 12:0612:06, 18 March 2014 diff hist +38 N Labeled AB staining Created page with "<h4>Pax7 + cy3 + NeuN – cy5Fab</h4>"
- 11:5611:56, 18 March 2014 diff hist +50 Overview →Protocols
10 March 2014
- 12:2112:21, 10 March 2014 diff hist +10 RNA formaldehyde gels No edit summary
- 12:1712:17, 10 March 2014 diff hist +649 RNA formaldehyde gels No edit summary
- 12:1112:11, 10 March 2014 diff hist +205 RNA formaldehyde gels No edit summary
- 12:0612:06, 10 March 2014 diff hist +5 RNA formaldehyde gels No edit summary
- 12:0512:05, 10 March 2014 diff hist +248 N RNA formaldehyde gels Created page with "<h3>Prepare gel box: <li>Clean gel box and combs etc with detergent, then dionized water. <li>Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored i..."
- 12:0112:01, 10 March 2014 diff hist +57 Overview →Molecular biology
- 08:5208:52, 10 March 2014 diff hist −45 RNA Extraction from axolotl tissue No edit summary
- 08:4908:49, 10 March 2014 diff hist +755 RNA Extraction from axolotl tissue No edit summary
- 08:4308:43, 10 March 2014 diff hist +41 RNA Extraction from axolotl tissue No edit summary
- 08:3908:39, 10 March 2014 diff hist +56 RNA Extraction from axolotl tissue No edit summary
- 08:3408:34, 10 March 2014 diff hist +239 RNA Extraction from axolotl tissue No edit summary
- 08:2008:20, 10 March 2014 diff hist −1 RNA Extraction from axolotl tissue No edit summary
- 08:2008:20, 10 March 2014 diff hist +8 RNA Extraction from axolotl tissue No edit summary
- 08:1808:18, 10 March 2014 diff hist +12 N RNA Extraction from axolotl tissue Created page with " Materials:"
6 March 2014
- 12:3612:36, 6 March 2014 diff hist −5 Overview →Molecular biology
- 12:2712:27, 6 March 2014 diff hist −3 Overview →Molecular biology
- 12:2212:22, 6 March 2014 diff hist +89 Overview →Molecular biology
- 12:1012:10, 6 March 2014 diff hist −102 Overview →RNA
- 09:3009:30, 6 March 2014 diff hist +93 RNA extraction from axolotl tissue →Procedure to extract total RNA: current
- 09:2109:21, 6 March 2014 diff hist +12 RNA extraction from axolotl tissue →Procedure to extract total RNA:
- 09:2009:20, 6 March 2014 diff hist +1,242 N RNA extraction from axolotl tissue Created page with "== RNA Extraction from axolotl tissue == === Materials: === <ul> <li>Trizol (Invitrogen)</li> <li>Chloroform</li> <li>Isopropanol</li> <li>RNase free water</li> <..."
- 08:3508:35, 6 March 2014 diff hist +95 Overview →Protocols