User contributions for Serghein
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29 November 2012
- 23:1023:10, 29 November 2012 diff hist +718 Overview No edit summary
- 20:2920:29, 29 November 2012 diff hist +1,231 Overview No edit summary
- 20:2520:25, 29 November 2012 diff hist +1,764 N Myoblasts electroporation Created page with "== Preparation == === (Start) Lamina === * Press bottom of ventilation * Switch on the light, put cable into the hood === (Finish) Lamina === * Press ventilation button unti..."
- 16:4716:47, 29 November 2012 diff hist +836 N Electroporation of nec Created page with "== Electroporation == # Incubate NSC 60-90min with 1mM DPBS/EDTA # Prepare 1.5ml tubes with DNA mix (max. 3µg/µl, 0.75-1µg/µl per plasmid). Max. 3µl DNA mixture # Prepare..."
- 16:4216:42, 29 November 2012 diff hist +499 N Electroporation of blastema cells Created page with "# Incubate Blastema Cells 20-30min with 1mM DPBS/EDTA # Prepare 1.5ml tubes with DNA mix (max. 3µg/µl, 0.75-1µg/µl per plasmid). Max. 3µl DNA mixture # Prepare 12well pla..."
- 16:4016:40, 29 November 2012 diff hist −1 Blastema cells No edit summary
- 16:3916:39, 29 November 2012 diff hist +550 N Blastema cells Created page with "== Composition for A1/BL cells == <ul> <li>250 ml MEM 21090</li> <li>100 ml H2O</li> <li>40 ml FCS for A1 cells</li> <li>4 ml pen/strep</li> <li>4 ml insulin</li> ..."
- 16:3416:34, 29 November 2012 diff hist +520 Overview No edit summary
- 16:1216:12, 29 November 2012 diff hist −1 Overview No edit summary
- 16:1216:12, 29 November 2012 diff hist +2 m Overview No edit summary
- 16:1016:10, 29 November 2012 diff hist −1 m Leica confocal microscope No edit summary current
- 16:0916:09, 29 November 2012 diff hist +1 m Leica confocal microscope No edit summary
- 16:0916:09, 29 November 2012 diff hist +117 Leica confocal microscope No edit summary
- 16:0616:06, 29 November 2012 diff hist +312 Overview No edit summary
- 16:0116:01, 29 November 2012 diff hist +843 Overview No edit summary
19 November 2012
- 11:0411:04, 19 November 2012 diff hist +1 Heat shock competent cells No edit summary current
- 11:0411:04, 19 November 2012 diff hist −2 Heat shock competent cells No edit summary
- 11:0211:02, 19 November 2012 diff hist +100 Heat shock competent cells No edit summary
- 10:0710:07, 19 November 2012 diff hist 0 Template:Tip No edit summary
- 10:0710:07, 19 November 2012 diff hist −6 Template:Tip No edit summary
- 10:0710:07, 19 November 2012 diff hist +11 Template:Tip No edit summary
17 November 2012
- 20:4020:40, 17 November 2012 diff hist +17 Ordering common stuff No edit summary current
- 20:4020:40, 17 November 2012 diff hist +181 N Ordering common stuff Created page with "== Responsible persons == If you have any troubles please contact Maritta or Kathleen. == Ordering list == Above Marittas desk is a folder, there she listed the common used ..."
- 20:3920:39, 17 November 2012 diff hist +465 N Vacuum pumps Created page with "== Responsible persons == If you have any troubles please contact Maritta or Kathleen. == Common problems == Please avoid sucking liquids into the pipe of the pump-system th..." current
- 20:3820:38, 17 November 2012 diff hist +322 N Dissecting microscopes Created page with "== Responsible persons == If you have any troubles please contact Maritta or Kathleen. == Replacing the bulbs == If the bulb burnt out and need to replace it by a new one, y..." current
- 20:3620:36, 17 November 2012 diff hist +2,264 N Heat shock competent cells Created page with "== Responsible persons == If you have any troubles please contact Annett or Akira. == TB buffer == === Ingredients === 10 mM HEPES pH 6.7, 15 mM CaCl2, 55 mM MnCl2, 250 mM KC..."
- 20:2620:26, 17 November 2012 diff hist +275 Leica confocal microscope No edit summary
- 20:2520:25, 17 November 2012 diff hist +1,407 Leica confocal microscope No edit summary
- 20:1820:18, 17 November 2012 diff hist +591 N Leica confocal microscope Created page with "== Responsible persons == If you have any troubles please contact Sven or Jan. == For beginners == If you like to work with the microscope please ask one of the responsible p..."
- 20:1620:16, 17 November 2012 diff hist +1 m Antibiotics No edit summary current
- 20:1520:15, 17 November 2012 diff hist −18 Template:Tip No edit summary
- 20:1520:15, 17 November 2012 diff hist +18 Template:Tip No edit summary
- 20:0420:04, 17 November 2012 diff hist −4 Template:Tip No edit summary
- 20:0420:04, 17 November 2012 diff hist 0 N File:Tip.png No edit summary current
- 20:0220:02, 17 November 2012 diff hist +109 Template:Tip No edit summary
- 20:0120:01, 17 November 2012 diff hist +13 N Template:Tip Created page with "NOTE: {{{1}}}"
- 19:5419:54, 17 November 2012 diff hist +62 Antibiotics No edit summary
- 19:5119:51, 17 November 2012 diff hist −1 Antibiotics No edit summary
- 19:5119:51, 17 November 2012 diff hist +1 Antibiotics No edit summary
- 19:5019:50, 17 November 2012 diff hist +149 N Antibiotics Created page with "== Responsible persons == If you have any troubles please contact Prayag or Walter. == Antibiotics list == Antibiotics"
- 19:4419:44, 17 November 2012 diff hist 0 N File:Antibiotics.png No edit summary current
- 19:4119:41, 17 November 2012 diff hist +1 m Autoclave No edit summary current
- 19:4119:41, 17 November 2012 diff hist +7 Autoclave No edit summary
- 19:4119:41, 17 November 2012 diff hist +293 Autoclave No edit summary
- 19:3919:39, 17 November 2012 diff hist +621 Autoclave No edit summary
- 19:3119:31, 17 November 2012 diff hist +608 Autoclave No edit summary
- 19:2219:22, 17 November 2012 diff hist +1,279 N Autoclave Created page with "== Responsible persons == If you have any troubles please contact Andrea or Volker. == Usage == Our autoclave has a storage tank for de-ionized water. Make sure that it is f..."
12 November 2012
- 16:5016:50, 12 November 2012 diff hist +986 N Cryostat Created page with "== Responsible persons == If you have any troubles please contact Kerstin or Eugen. == Rules == <span style="color:red"><b>If it’s your first time using the cryostat, plea..." current
- 16:4016:40, 12 November 2012 diff hist +1 Overview No edit summary
- 16:4016:40, 12 November 2012 diff hist +1 Overview No edit summary