For scRNA-seq

scRNA-seq

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(Continuing directly from the dissociation protocol or from FACS)

• Resuspend the cell pellet with 150-300uL AMEM(0) depending on the size of the pellet.
• Immediately sort the cells into 1.5mL tube with 700uL AMEM(0).
  • Normally we use F02 sorter because it has lasers for both GFP and mCherry.
• Note the FACS count.
• Centrifuge 300xrcf, 20 degree, 5 minutes. Remove supernatant.

• The sample is ready for downstream applications:

-> scRNA-seq

-> cell transplantation