G418 medium preparation

Materials

• 2x 15 ml FALCON tube
• filter 0.22 µl
• 5 ml syringe
• DMEM
• G418

Procedure

1. 1 g of weight has a total concentration from 0,751 g
2. How much of DMEM we need for 1,5 g G418 if we prepare a stock solution with a concentration of 200 mg/ ml?
3. 1.5g powder has a total concentration from 1,1256 g G418

1125,6 mg/ 200 mg = 5,6

it means, that you need 5,6 ml DMEM for 1,5 g G418 , if you wanted a concentration of 200 mg/ ml

dissolve the powder in DMEM

if it is dissolve than filter the solution: put the pump from the syringe out, fill the antibiotic in the syringe, take the pump back and press all across the filter

label the vessel with the name of the antibiotics and the stock solution f.e. G418 200 mg/ ml in DMEM

for using:

HS with antibiotics: 400 µg/ ml

0,2/ 0,0004 = 500 ⇒ 100 ml media/ 500 ⇒ 0,2 ml antibiotics in 1200 ml media