Preparation of High Serum for C2C12 cells

Materials:

- 1 tube pen/strep (green, 4 ml) - 1 tube glutamine (blue, 4 ml) → this is not necessary if the DMEM has glutamax - 1 bottle DMEM with 4500 mg/ l glucose - 40 ml FCS (frozen in a 50 ml FALCON tube) - 1 large Stericup (500 ml) (bottle + filter + top)

procedure:

- thaw all frozen stuffs at 37 ºC - add in the Stericup the 40 ml FCS, 4 ml pen/ strep and the 4 ml glutamine (if necessary) - fill this up to 400 ml with the DMEM - filtrate the Medium in the bottle by connecting the vacuum pump to the steritop - label the bottle with the name, the date and “high serum for C2C12”