Thawing frozen C2C12 cells

Materials:

- frozen C2C12 cells - HS for C2C12 cells - pipettes and tips - FALCON tube 15 ml - Cell culture flask

Procedure:

- heat the HS up to 37ºC - pipette 14 ml C2C12 HS media in a 75 cm2 cell culture flask - pipette carefully 1 ml from the warm media in the cryo tube to the frozen cells - pipette carefully up and down till the cells are thaw - pipette all in the FALCON tube - centrifuge at 1000 xg, 3 min at 4ºC - label the cell culture flask with the name, date and C2C12 with the passage number - aspirate of the media and resuspend the cells in 1 ml of the new HS - pipette the suspension in the cell culture flask - check the cells under the microscope - put the flask at 37ºC, 10% CO2