Purifying IgG1 from Hybridoma Supernatant

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Revision as of 09:25, 24 March 2014 by Stephanh (talk | contribs) (Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ...")
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Materials:

  • Supernatant (100-200 ml)
  • Peristaltic pump
  • 1 ml HiTrap ProteinG column (binding capacity = 25 ml)
  • PBS (100 ml)
  • 10x PBS
  • .1 M glycine pH 2.7 (25 ml)
  • 1 M Tris pH 9.0 (10 ml)
  • 10 Eppendorf tubes
  • 20% ETOH/H2O (25 ml)
    • Retrieved from "https://wiki.tanakalab.org/index.php?title=Purifying_IgG1_from_Hybridoma_Supernatant&oldid=336"