Double Immunostaining via antigen retrieval

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Revision as of 15:00, 19 February 2013 by Sarahh (talk | contribs) (Created page with "# Fix the tissue for 4 hours @ RT in 4% MEMFA (Made in water) # Wash the tissue with PBS 3x10' @ RT on a shaker # Put in 30% sucrose and shake O/N at 4˚C (cold room) # Embed ...")
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  1. Fix the tissue for 4 hours @ RT in 4% MEMFA (Made in water)
  2. Wash the tissue with PBS 3x10' @ RT on a shaker
  3. Put in 30% sucrose and shake O/N at 4˚C (cold room)
  4. Embed in OCT and freeze on Dry ice and transfer the frozen block to -20˚C freezer until sectioning
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