For FACS

Finish the dissociation protocol
Resuspend in 150-200uL AMEM(0)
Send to FACS facility immediately
- Book FACS machine F02 because it has both GFP and mCherry lasers.
- Normal setting: 85um nozzle with 0.7x FACS Flow for frog cells; 100um nozzle with 0.7x FACS Flow for axolotl cells.
Collect the cells into a 1.5mL tube with 700uL AMEM(0) inside. Note down the FACS count.
Spin down the cells. 300xrcf, 20 degree, 5 minutes.
Resuspend in 50-100uL AMEM(0)
Count the cells

The sample is ready for downstream applications:
- scRNA-seq
- cell transplantation

With FACS