LiberaseTM dissociation

Preparing reagents

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0.7x Phosphate Buffer Saline (PBS)

• 1x PBS 35 mL
• dH2O 15 mL
• Total 50 mL

Store at room temperature. Chill on ice before use.

Stock Liberase TM (26 Wünsch Unit (WU)/mL)

• Liberase TM 50mg
• dH2O 10mL
• Store in 50uL aliquots at -80 degree