Preparation of Freezing media for A1 cells

Materials

• DMSO (one ampules from Sigma)
• High serum for A1 cells
• 2x 50 ml FALCON tubes
• 1x 15 ml FALCON tube
• Pipettes
• 10x 5 ml FALCON tubes

Procedure

- clean the ampuls with ethanol - transfer the DMSO into the 15 ml FALCON tube - pipette in each 50 ml FALCON tube 18 ml High serum - put into each 2 ml DMSO and mix - aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube) - store the tubes into –20ºC