User contributions for Stephanh

For Stephanh talk block log uploads logs

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08:2708:27, 7 May 2014 diff hist +943‎ N Antibody staining ‎ Created page with "* Cut the tail and fix it in fresh 4% PFA in phosphate buffer from few hours to o/n always at 4C * Wash in PBS 1 hour at room temperature, put tail in 30% sucrose Put in OCT s..."

09:0009:00, 2 May 2014 diff hist +252‎ BrdU labelling of A1 cells in 96-well plate ‎No edit summary
08:5608:56, 2 May 2014 diff hist +102‎ N BrdU labelling of A1 cells in 96-well plate ‎ Created page with "== Materials: == * 5ml tube * SF MEM * BrDU drawer no. 3 * Multipipette * Pipette 100ul * Pipette 5ml"

08:4608:46, 28 April 2014 diff hist 0‎ Passaging C2C12 cells ‎No edit summary
08:4508:45, 28 April 2014 diff hist 0‎ Passaging C2C12 cells ‎No edit summary
08:3608:36, 28 April 2014 diff hist 0‎ Passaging C2C12 cells ‎No edit summary
08:3408:34, 28 April 2014 diff hist 0‎ Passaging C2C12 cells ‎No edit summary
08:3208:32, 28 April 2014 diff hist 0‎ Passaging C2C12 cells ‎No edit summary
08:2908:29, 28 April 2014 diff hist +805‎ Passaging C2C12 cells ‎No edit summary
08:2808:28, 28 April 2014 diff hist +17‎ Passaging C2C12 cells ‎No edit summary
06:5806:58, 28 April 2014 diff hist +143‎ N Passaging C2C12 cells ‎ Created page with "== Materials: == * 1x 15ml FALCON tube * PBS w/o Ca2+ and Mg2+ * TE in PBS (4 ml TE + 36 ml PBS) * HS for C2C12 cells * pipettes * 75 cm2 flask"

09:3209:32, 24 March 2014 diff hist +768‎ Purifying IgG1 from Hybridoma Supernatant ‎No edit summary
09:2709:27, 24 March 2014 diff hist −45‎ Purifying IgG1 from Hybridoma Supernatant ‎No edit summary
09:2509:25, 24 March 2014 diff hist +313‎ N Purifying IgG1 from Hybridoma Supernatant ‎ Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ..."

12:4812:48, 18 March 2014 diff hist −6‎ Overview ‎ →‎Protocols
12:4512:45, 18 March 2014 diff hist +6‎ Overview ‎ →‎Antibody
12:4212:42, 18 March 2014 diff hist +133‎ Overview ‎ →‎Protocols
12:1912:19, 18 March 2014 diff hist +861‎ Labeled AB staining ‎No edit summary
12:1212:12, 18 March 2014 diff hist +315‎ Labeled AB staining ‎No edit summary
12:0612:06, 18 March 2014 diff hist +38‎ N Labeled AB staining ‎ Created page with "<h4>Pax7 + cy3 + NeuN – cy5Fab</h4>"
11:5611:56, 18 March 2014 diff hist +50‎ Overview ‎ →‎Protocols

12:2112:21, 10 March 2014 diff hist +10‎ RNA formaldehyde gels ‎No edit summary
12:1712:17, 10 March 2014 diff hist +649‎ RNA formaldehyde gels ‎No edit summary
12:1112:11, 10 March 2014 diff hist +205‎ RNA formaldehyde gels ‎No edit summary
12:0612:06, 10 March 2014 diff hist +5‎ RNA formaldehyde gels ‎No edit summary
12:0512:05, 10 March 2014 diff hist +248‎ N RNA formaldehyde gels ‎ Created page with "<h3>Prepare gel box: <li>Clean gel box and combs etc with detergent, then dionized water. <li>Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored i..."
12:0112:01, 10 March 2014 diff hist +57‎ Overview ‎ →‎Molecular biology
08:5208:52, 10 March 2014 diff hist −45‎ RNA Extraction from axolotl tissue ‎No edit summary
08:4908:49, 10 March 2014 diff hist +755‎ RNA Extraction from axolotl tissue ‎No edit summary
08:4308:43, 10 March 2014 diff hist +41‎ RNA Extraction from axolotl tissue ‎No edit summary
08:3908:39, 10 March 2014 diff hist +56‎ RNA Extraction from axolotl tissue ‎No edit summary
08:3408:34, 10 March 2014 diff hist +239‎ RNA Extraction from axolotl tissue ‎No edit summary
08:2008:20, 10 March 2014 diff hist −1‎ RNA Extraction from axolotl tissue ‎No edit summary
08:2008:20, 10 March 2014 diff hist +8‎ RNA Extraction from axolotl tissue ‎No edit summary
08:1808:18, 10 March 2014 diff hist +12‎ N RNA Extraction from axolotl tissue ‎ Created page with " Materials:"

12:3612:36, 6 March 2014 diff hist −5‎ Overview ‎ →‎Molecular biology
12:2712:27, 6 March 2014 diff hist −3‎ Overview ‎ →‎Molecular biology
12:2212:22, 6 March 2014 diff hist +89‎ Overview ‎ →‎Molecular biology
12:1012:10, 6 March 2014 diff hist −102‎ Overview ‎ →‎RNA
09:3009:30, 6 March 2014 diff hist +93‎ RNA extraction from axolotl tissue ‎ →‎Procedure to extract total RNA: current
09:2109:21, 6 March 2014 diff hist +12‎ RNA extraction from axolotl tissue ‎ →‎Procedure to extract total RNA:
09:2009:20, 6 March 2014 diff hist +1,242‎ N RNA extraction from axolotl tissue ‎ Created page with "== RNA Extraction from axolotl tissue == === Materials: === <ul> <li>Trizol (Invitrogen)</li> <li>Chloroform</li> <li>Isopropanol</li> <li>RNase free water</li> <..."
08:3508:35, 6 March 2014 diff hist +95‎ Overview ‎ →‎Protocols

11:3711:37, 17 January 2013 diff hist +7‎ Overview ‎ →‎Artemia Hatching Preparation: Protocol for 15 Ltr.
11:3511:35, 17 January 2013 diff hist −1‎ Overview ‎ →‎Artemia Hatching Preparation: Protocol for 15 Ltr.
11:3411:34, 17 January 2013 diff hist +370‎ Overview ‎ →‎Artemia Hatching Preparation: Protocol for 15 Ltr.
11:2911:29, 17 January 2013 diff hist +62‎ Overview ‎ →‎Artemia (Brine shrimp eggs)
11:1011:10, 17 January 2013 diff hist +78‎ Overview ‎No edit summary
11:0011:00, 17 January 2013 diff hist −37‎ Overview ‎No edit summary
10:5910:59, 17 January 2013 diff hist +37‎ Overview ‎No edit summary

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