LiberaseTM dissociation: Difference between revisions
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'''0.7x Phosphate Buffer Saline (PBS)''' | |||
1x PBS 35 mL | *1x PBS 35 mL | ||
dH2O 15 mL | *dH2O 15 mL | ||
Total 50 mL | *Total 50 mL | ||
Store at room temperature. Chill on ice before use. | *Store at room temperature. Chill on ice before use. | ||
'''Stock Liberase TM (26 Wünsch Unit (WU)/mL)''' | '''Stock Liberase TM (26 Wünsch Unit (WU)/mL)''' | ||
Liberase TM 50mg | *Liberase TM 50mg | ||
dH2O 10mL | *dH2O 10mL | ||
Store in 50uL aliquots at -80 degree | *Store in 50uL aliquots at -80 degree | ||
Revision as of 20:19, 18 July 2022
Preparing reagents
0.7x Phosphate Buffer Saline (PBS)
- 1x PBS 35 mL
- dH2O 15 mL
- Total 50 mL
- Store at room temperature. Chill on ice before use.
Stock Liberase TM (26 Wünsch Unit (WU)/mL)
- Liberase TM 50mg
- dH2O 10mL
- Store in 50uL aliquots at -80 degree