Preparation of Freezing media for A1 cells: Difference between revisions
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(Created page with "Materials * DMSO (one ampules from Sigma) * High serum for A1 cells * 2x 50 ml FALCON tubes * 1x 15 ml FALCON tube * Pipettes ** 10x 5 ml FALCON tubes Procedure - clean the a...") |
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| Line 1: | Line 1: | ||
Materials | == Materials == | ||
* DMSO (one ampules from Sigma) | * DMSO (one ampules from Sigma) | ||
* High serum for A1 cells | * High serum for A1 cells | ||
| Line 5: | Line 5: | ||
* 1x 15 ml FALCON tube | * 1x 15 ml FALCON tube | ||
* Pipettes | * Pipettes | ||
* 10x 5 ml FALCON tubes | |||
Procedure | == Procedure == | ||
- clean the ampuls with ethanol | - clean the ampuls with ethanol | ||
- transfer the DMSO into the 15 ml FALCON tube | - transfer the DMSO into the 15 ml FALCON tube | ||
Revision as of 12:54, 17 April 2015
Materials
- DMSO (one ampules from Sigma)
- High serum for A1 cells
- 2x 50 ml FALCON tubes
- 1x 15 ml FALCON tube
- Pipettes
- 10x 5 ml FALCON tubes
Procedure
- clean the ampuls with ethanol - transfer the DMSO into the 15 ml FALCON tube - pipette in each 50 ml FALCON tube 18 ml High serum - put into each 2 ml DMSO and mix - aliquot the freezing media into the 5 ml FALCON tubes (4 ml per tube) - store the tubes into –20ºC