RNA Extraction from axolotl tissue: Difference between revisions
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Trizol (Invitrogen)
Chloroform
Isopropanol
RNase free water
Potter homogenizer plus appropriate accessories (Stoessel und Pistille)
5 ml syringe
25 guage needle
Autoclaved Eppendorf tubes
75% ethanol in DEPC water
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<h3>Materials:</h3> | <h3>Materials:</h3> | ||
<li>Trizol (Invitrogen)</li> | <li>Trizol (Invitrogen)</li> | ||
<li>Chloroform</li> | |||
<li>Isopropanol</li> | |||
<li>RNase free water</li> | |||
<li>Potter homogenizer plus appropriate accessories (Stoessel und Pistille)</li> | |||
<li>5 ml syringe</li> | |||
<li>25 guage needle</li> | |||
<li>Autoclaved Eppendorf tubes</li> | |||
<li>75% ethanol in DEPC water</li> | |||