LiberaseTM dissociation: Difference between revisions

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'''0.7x Phosphate Buffer Saline (PBS)'''
<li>'''0.7x Phosphate Buffer Saline (PBS)'''<\li>
1x PBS 35 mL
1x PBS 35 mL
dH2O 15 mL
dH2O 15 mL

Revision as of 20:18, 18 July 2022

Preparing reagents


  • 0.7x Phosphate Buffer Saline (PBS)<\li> 1x PBS 35 mL dH2O 15 mL Total 50 mL Store at room temperature. Chill on ice before use. Stock Liberase TM (26 Wünsch Unit (WU)/mL) Liberase TM 50mg dH2O 10mL Store in 50uL aliquots at -80 degree