Thawing frozen C2C12 cells: Difference between revisions

Revision as of 08:35, 27 March 2015

heat the HS up to 37ºC
pipette 14 ml C2C12 HS media in a 75 cm2 cell culture flask
pipette carefully 1 ml from the warm media in the cryo tube to the frozen cells
pipette carefully up and down till the cells are thaw
pipette all in the FALCON tube
centrifuge at 1000 xg, 3 min at 4ºC
label the cell culture flask with the name, date and C2C12 with the passage number
aspirate of the media and resuspend the cells in 1 ml of the new HS
pipette the suspension in the cell culture flask
check the cells under the microscope
put the flask at 37ºC, 10% CO2

@@ Line 7: / Line 7: @@
 == Procedure ==
-* heat the HS up to 37ºC
+# heat the HS up to 37ºC
-* pipette 14 ml C2C12 HS media in a 75 cm2 cell culture flask
+# pipette 14 ml C2C12 HS media in a 75 cm2 cell culture flask
-* pipette carefully 1 ml from the warm media in the cryo tube to the frozen cells
+# pipette carefully 1 ml from the warm media in the cryo tube to the frozen cells
-* pipette carefully up and down till the cells are thaw
+# pipette carefully up and down till the cells are thaw
-* pipette all in the FALCON tube
+# pipette all in the FALCON tube
-* centrifuge at 1000 xg, 3 min at 4ºC
+# centrifuge at 1000 xg, 3 min at 4ºC
-* label the cell culture flask with the name, date and C2C12 with the passage number
+# label the cell culture flask with the name, date and C2C12 with the passage number
-* aspirate of the media and resuspend the cells in 1 ml of the new HS
+# aspirate of the media and resuspend the cells in 1 ml of the new HS
-* pipette the suspension in the cell culture flask
+# pipette the suspension in the cell culture flask
-* check the cells under the microscope
+# check the cells under the microscope
-* put the flask at 37ºC, 10% CO2
+# put the flask at 37ºC, 10% CO2