Electroporation of A1 cells: Difference between revisions
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| Line 30: | Line 30: | ||
|4.6 mM | |4.6 mM | ||
|Tris pH 7.8-8.0 | |Tris pH 7.8-8.0 | ||
|5.75 ml (2M) | |5.75 ml (2M)|} | ||
== Procedure == | == Procedure == | ||
Revision as of 09:37, 25 March 2015
Materials
- Ice
- 4 mM cuvettes, N+1 cuvettes
- Steinberg's, at 4 C.
- Square pulse electroporator (we use the BTX 830 Squarporator)
Steinberg's
| 1x | FW (MW) | 5x/500 ml |
|---|---|---|
| 58 mM NaCl | 58.44 | 8.47 g |
| 0.67 mM KCl | 74.56 | 1.68 ml (1M) |
| 0.44 mM Ca(NO3) | 236.20 | 1.1 ml (1M) |
| 1.3 mM MgSO4 | 246.47 | 3.25 ml (1M) |
| 4.6 mM | Tris pH 7.8-8.0 | }
Procedure
|