Purifying IgG1 from Hybridoma Supernatant: Difference between revisions
Jump to navigation
Jump to search
Supernatant (100-200 ml)
Peristaltic pump
1 ml HiTrap ProteinG column (binding capacity = 25 ml)
PBS (100 ml)
10x PBS
.1 M glycine pH 2.7 (25 ml)
1 M Tris pH 9.0 (10 ml)
10 Eppendorf tubes
20% ETOH/H2O (25 ml)
(Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ...") |
No edit summary |
||
| Line 1: | Line 1: | ||
<h3>Materials:</h3> | <h3>Materials:</h3> | ||
<li>Supernatant (100-200 ml) | <li>Supernatant (100-200 ml) | ||
<li>Peristaltic pump | <li>Peristaltic pump | ||
<li>1 ml HiTrap ProteinG column (binding capacity = 25 ml) | <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml) | ||
<li>PBS (100 ml) | <li>PBS (100 ml) | ||
<li>10x PBS | <li>10x PBS | ||
<li>.1 M glycine pH 2.7 (25 ml) | <li>.1 M glycine pH 2.7 (25 ml) | ||
<li>1 M Tris pH 9.0 (10 ml) | <li>1 M Tris pH 9.0 (10 ml) | ||
<li>10 Eppendorf tubes | <li>10 Eppendorf tubes | ||
<li>20% ETOH/H2O (25 ml) | <li>20% ETOH/H2O (25 ml) | ||